Volume 3, Issue 2

Modulation of Mitogen-Response and Phagocytic Activities of Human Lymphocytes and Macrophages by Aqueous, Hexane and Methanol Extracts of Stem Bark of Boswellia Dalzeilii, Hutch (Family: Burseraceae)

Author

OUMAR Mahamat1,3*, TUME Christopher2 and KAMANYI Albert3

Abstract

The barks of Boswellia dalzeilii (B. dalzeilii) have been traditionally used in Cameroon and other Africa countries. The aqueous extract (Aq-extract), methanol extract (Me-extract) and hexane extract (He-extract) of Boswellia dalzeilii barks were evaluated for immune modulatory activity using in vitro and in vivo methodologies. Effect of the extracts was evaluated at various concentrations (2560 – 160 μg/ml) for reduction of Nitro blue tetrazolium (NBT), inducible nitric oxide synthase (iNOS) and myeloperoxidase activity of macrophages. Particularly, aq-extract was evaluated in vivo for carbon clearance. The Aq-extract that evaluated for in vivo phagocytic activity by carbon clearance assay in mice, showed significant increase in the phagocytic index at 0.75, 1.25 and 2.5μg/kg dose. The extract showed stimulation reduction of NBT, nitric oxide production after blocking of iNOS using NG-nitro-L-arginine methyl ester (L-NAME), and myeloperoxidase activity. The effect of the extract on lymphocyte proliferation and IL-2 production was evaluated. B.dalzeilii showed a significant effect on lymphocyte proliferation and IL-2 production. The results suggest that B. dalzeilii stimulated non-specific murine immune system, both in vitro and in vivo.

DOI

https://doi.org/10.62226/ijarst20140220

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