Volume 7, Issue 1

Studies on optimization of growth parameters for enhanced production of antibiotic activity by isolated Marineactinomycete.

Author

M. V. K. Narsingarao*, T. Samuel David, N. Nagaraju, G. Ravisankar and T. Saroja

Abstract

Abstract:

The aim of the present study is to optimize the growth conditions for improved production of antimicrobial activity by promising marine actinomycetes isolated from marine sediments. The indigenous isolate KRBT-401was then investigated for the effect of different nutrients and cultural conditions for antibiotic production. Pridham and Gottlieb's inorganic salts medium was used as the production medium base. The carbon sources employed are arabinose, fructose, galactose, glucose, glycerol, lactose, maltose, mannitol, meso-inositol, starch, sucrose and xylose at 1% (w/v). Among the various carbon sources studied, glucose produced more antibiotic yield followed by starch and glycerol. Similarly the effect of various organic nitrogen sources, inorganic nitrogen sources and aminoacids was studied on the production of antibiotic. Ammonium nitrate at a concent ration of 3.0g/L was found to be the suitable concentration for maximum antibiotic yield. By performing studies on various physiological parameters, nutrients and trace metal ions that affect antibiotic yield, a synthetic medium was formulated for the maximum production of antibiotic under shake flask culture by KRBT-401. The antibiotic productivity employing optimized fermentation conditions (285μg/ml) was 260% higher than the antibiotic yield using initial fermentation conditions (115μg/ml).

DOI

https://doi.org/10.62226/ijarst20180125

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