Volume 5, Issue 2

Analysis of bioactive compounds and antimicrobial screening of Phyllanthus amarus Schum. and Thonn.

Author

S. S. Sravanthi Pammi, T. V. S. Padmavathi and Archana Giri*

Abstract

Abstract:

Phytochemical screening of the extracts in different parts of Phyllanthus amarus revealed the presence of alkaloids, flavonoids, tannins, saponins, steroids and terpenoids. The presence of various bioactive compounds in the plant parts of Phyllanthus amarus helps us to evaluate growth effects of some selected bacteria. The development of resistance against conventional antibiotics by pathogens is of great concern. Leaf, stem and root of Phyllanthus amarus were separately extracted with 70 % ethanol and 70 % methanol and the crude extracts were assayed for six pathogenic microorganism’s viz. Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Proteus mirabilis for susceptibility to the various extracts of P. amarus in vitro. The zones of inhibition of both plant extracts ranged between 7.5- 30.0 mm against the test organisms. Leaf extracts of Phyllanthus amarus were most active against the growth of the bacteria. S. aureus was the most susceptible organism and the most resistant bacterium was P. aeruginosa to the ethanolic extracts basing on MIC values obtained for leaf,stem and root parts of P.amarus.. Ciprofloxacin and Chloramphenicol were the standard antibiotics that were used. Antimicrobial study suggests that both methanolic and ethanolic extracts of the plant show approximately the same inhibitory effect on the test pathogenic microorganisms. Hence Ethanolic and methanolic leaf extracts of P. amarus were subjected to GC-MS analysis to investigate possible bioactive components in them. Nine compounds were detected in ethanolic leaf extract and seventeen medicinally important bioactive compounds were detected in methanolic leaf extract which confirms the application of P. amarus for finding novel drug compound.

DOI

https://doi.org/10.62226/ijarst20160280

PAGES : 595-603 | 94 VIEWS | 43 DOWNLOADS

Download Full Article